Genetic analysis of the right (3′) end of the rosy locus inDrosophila melanogaster

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Abstract
SUMMARY: Prior reports from this laboratory have described the experimental basis for our understanding of the rosy locus (ry: 3–52·0) ofDrosophila melanogasteras a bipartite genetic entity consisting of a structural element that codes for the xanthine dehydrogenase (XDH) peptide and a contiguouscis-acting control element immediately to the left of the structural element. Although the left end (5′) of the structural element has been well defined, the right boundary (3′) has been given only casual treatment in our prior reports. In our recent studies of rosy locus expression we have been concerned with the production and identification of mutations in the non-structural regions immediately flanking the structural element. An improved definition of the right end of the structural element is essential to this analysis. In addition to producing a better definition of the right boundary of the structural element, this study produced several phenotypically novel mutations. These mutations were classified initially ascontrol element mutations, but upon analysis were found to map within the rosy structural element.Noevidence was obtained for the existence of a control element contiguous with the right end of the structural element.