Liposomal transfection of human γ-interferon gene into human glioma cells and adoptive immunotherapy using lymphokine-activated killer cells

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Abstract
✓ The authors evaluated the effect of liposomal transfection of human γ-interferon (HuIFN-γ) gene into human glioma cells and lymphokine-activated killer (LAK) cells, alone and in combination. An HuIFN-γ; gene inserted in a eukaryotic expression vector was entrapped in liposomes bearing positive surface charges. Liposomal gene transfection induced production of HuIFN-γ and its secretion in culture medium of human glioma cell lines (SK-MG-1 and U-251 MG). At 4 days after transfection, the cells produced 10 to 50 U/ml of HuIFN-γ in the medium, whereby the major histocompatibility complex (MHC) class I and II antigens, as well as intercellular adhesion molecule-1 (ICAM-1), were induced on the glioma cell surface. The growth-inhibiting effect of transfection-induced HuIFN-γ; was much stronger in comparison with control cultures exposed to 500 U/ml of exogenously added HuIFN-γ;. In addition, 20% to 40% growth inhibition was obtained in the glioma cells when they were treated with LAK cells alone at a 5:1 ratio...