Distribution of endogenous type B and type D sheep retrovirus sequences in ungulates and other mammals.
- 16 April 1996
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 93 (8) , 3297-3302
- https://doi.org/10.1073/pnas.93.8.3297
Abstract
The jaagsiekte sheep retrovirus (JSRV), which appears to be a type B/D retrovirus chimera, has been incriminated as the cause of ovine pulmonary carcinoma. Recent studies suggest that the sequences related to this virus are found in the genomes of normal sheep and goats. To learn whether there are breeds of sheep that lack the endogenous viral sequences and to study their distribution among other groups of mammals, we surveyed several domestic sheep and goat breeds, other ungulates, and various mammal groups for sequences related to JSRV. Probes prepared from the envelope (SU) region of JSRV and the capsid (CA) region of a Peruvian type D virus related to JSRV were used in Southern blot hybridization with genomic DNA followed by low- and high-stringency washes. Fifteen to 20 CA and SU bands were found in all members of the 13 breeds of domestic sheep and 6 breeds of goats tested. There were similar findings in 6 wild Ovis and Capra genera. Within 22 other genera of Bovidae including domestic cattle, and 7 other families of Artiodactyla including Cervidae, there were usually a few CA or SU bands at low stringency and rare bands at high stringency. Among 16 phylogenetically distant genera, there were generally fewer bands hybridizing with either probe. These results reveal wide-spread phylogenetic distribution of endogenous type B and type D retroviral sequences related to JSRV among mammals and argue for further investigation of their potential role in disease.Keywords
This publication has 14 references indexed in Scilit:
- Analysis of a Type D Retroviral Capsid Gene Expressed in Ovine Pulmonary Carcinoma and Present in Both Affected and Unaffected Sheep GenomesVirology, 1994
- Enzootic nasal tumour of goats: demonstration of a type D-realated retrovirus in nasal fluids and tumoursJournal of General Virology, 1991
- Some characteristics of a retrovirus isolated from transformed bovine cellsVirology, 1989
- The generation of radiolabeled DNA and RNA probes with polymerase chain reactionAnalytical Biochemistry, 1989
- The etiology and pathogenesis of ovine pulmonary carcinoma (sheep pulmonary adenomatosis)Veterinary Microbiology, 1988
- Current perspectives in the biology of mouse mammary tumour virusVirus Research, 1987
- Nucleotide sequence of Mason-Pfizer monkey virus: An immunosuppressive D-type retrovirusCell, 1986
- Organization of the endogenous proviruses of chickens: Implications for origin and expressionVirology, 1981
- Endogenous mammary tumour virus DNA varies among wild mice and segregates during inbreedingNature, 1979
- Skunks have gene sequences in their cellular DNA related to squirrel monkey retrovirus: Transmission between species of a New World primate endogenous type D retrovirusBiochemical and Biophysical Research Communications, 1978