Immunohistochemical demonstration of β‐enolase in human skeletal muscle

Abstract
.beta.-Enolase activity was localized in human skeletal muscle fiber using the immunohistochemical method (2-step method). The 1st immunoreagent was rabbit anti-human .beta.-enolase serum raised in New Zealand white rabbit, and the 2nd was peroxidase conjugated staphylococcal protein A. The immunohistochemical reaction for .beta.-enolase was noted higher in type 2 fiber, which demonstrates the low oxidative and high glycolytic enzyme activity, than in type 1 fiber.