Results of a Cooperative Study Comparing the Precision of Peak Height and Area Measurements in Liquid Chromatography. Part II

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Abstract
Presented are the results from a second cooperative test comparing the precision of peak area and height measurements in isocratic liquid chromatography with UV detection. Forty-two laboratories participated in the current study in which the precision of quantitation for peak areas was compared with that for electronic and manual peak heights with a calibration standard and two four-component solutions. This program differed from previous studies in several respects. First, care was taken to ensure that all four components of the test solutions were well resolved from each other and from baseline perturbations. Second, the concentration range of the components in the test solutions was limited to a factor of five, and all peaks in a single solution were approximately the same size. Finally, comparisons between electronic and manual peak heights were made. In this study peak area measurements were consistently more precise and had less bias than height measurements. No significant differences in precision were noted between the two types of height measurements. These results suggest that peak area measurements are the preferred method of quantitation in isocratic liquid chromatography for well-resolved peaks. The relative standard deviation values for area measurements demonstrate that good inter-laboratory agreement will be possible with liquid chromatographic analyses.

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