Embryo Elongation and Germination Rates as Sensitive Indicators of Lettuce Seed Quality: Priming and Aging Studies

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Abstract
High-quality seed lots are required for successful establishment of horticultural crops. Testing methods are needed that can detect the early phases of aging prior to a significant loss in germination. Quality was assessed using both germination speed and uniformity on non-primed and primed, as well as non-aged and aged, lettuce (Lactuca sativa L.) seed. Speed and uniformity were quantified using time to 50% (T50) germination and one standard deviation (Tsd), respectively. Embryo elongation was developed as a rapid test by first soaking seeds for 2 hours, then cutting and removing the distal one-third of the seed, and finally observing the percentage of visibly elongated embryos from the seed coverings over time. The mild aging conditions employed in this study (45 °C and 50% relative humidity for up to 21 days) had little influence on viability (germination in all treatments was >98%), but reduced germination rate (increased the time for T50 and Tsd). Primed seeds aged faster than non-primed seeds under the same aging conditions. The percentage of elongated embryos was calculated at hourly intervals after cutting, and treatment differences were observed after a total hydration period of 5 hours. The embryo elongation test detected aging in both primed and non-primed treatments.

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