The Theory of Preparative Linear Chromatography: The Role of the Column Length

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Abstract
The effect of the column length, pressure drop across the column and the particle diameter on the preparative efficiency has been investigated for both the elution development and frontal analysis chromatography. It is found that, when all other variables are kept constant, the column should generally be as short as possible. However, when operating a column at the maximum flow velocity, the preparative efficiency increases almost linearly with the column length. The pressure drops required to yield this velocity are not unduly large and decrease rapidly as the particle diameter is increased which, however, can seriously impair the preparative efficiency.

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