Requirement for GTP hydrolysis in the formation of secretory vesicles
- 1 September 1990
- journal article
- Published by Springer Nature in Nature
- Vol. 347 (6289) , 207-208
- https://doi.org/10.1038/347207a0
Abstract
The specificity of vesicular transport in a cell is determined by the formation of vesicles with specific contents from a donor compartment and their selective fusion with the appropriate acceptor compartment. Several of the latter fusion steps have been investigated in detail using cell-free systems, and work with these systems as well as genetic evidence has revealed a role for GTP-binding proteins in membrane fusion processes. We have reconstituted the formation of constitutive secretory vesicles and immature secretory granules from the trans Golgi network in a cell-free system. We show here that the budding of both types of post-Golgi vesicles is inhibited by non-hydrolysable analogues of GTP, which suggests a more widespread role for GTP-binding proteins in membrane traffic than previously assumed.Keywords
This publication has 16 references indexed in Scilit:
- Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant.The Journal of cell biology, 1988
- Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitroCell, 1988
- Reconstitution of SEC gene product-dependent intercompartmental protein transportCell, 1988
- The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machineryCell, 1988
- Involvement of GTP-binding “G” proteins in transport through the Golgi stackCell, 1987
- Semi-intact cells permeable to macromolecules: Use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complexCell, 1987
- A ras-like protein is required for a post-Golgi event in yeast secretionCell, 1987
- Fusion between endocytic vesicles in a cell-free system.Proceedings of the National Academy of Sciences, 1987
- Reconstitution of an endocytic fusion event in a cell-free systemCell, 1985
- Reconstitution of the transport of protein between successive compartments of the golgi measured by the coupled incorporation of N-acetylglucosamineCell, 1984