CHEMICAL CONTROL OF VASCULAR CAMBIUM INITIATION IN ISOLATED RADISH ROOTS

Abstract
Vascular cambia were induced chemically in excised ""White Icicle" radish roots grown in sterile culture on a defined medium. Seedling roots which had been depleted of endogenous substances by 1 subculture period after excision underwent marked thickening when both kinin (at 1 mg/liter) and auxin (at 10-5 [image]) were supplied together with sucrose via the base of the root. Myo-inositol stimulated root growth and greatly promoted the thickening. The duration and longitudinal extent of the cambial activity was limited, in these experiments, probably because some factors, as yet unidentified, became deficient.