Pyrroloquinoline‐quinone: a reactive oxygen species scavenger in bacteria
- 4 November 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 578 (1-2) , 26-30
- https://doi.org/10.1016/j.febslet.2004.10.061
Abstract
Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with reactive oxygen species with rate constants comparable to other well known antioxidants, producing non-reactive molecular products. PQQ also protected plasmid DNA and proteins from the oxidative damage caused by gamma-irradiation in solution. The data suggest that radioprotective/oxidative stress protective ability of PQQ in bacteria may be consequent to scavenging of reactive oxygen species per se and induction of other free radical scavenging mechanism.Keywords
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