Effects of okadaic acid on the activities of two distinct phosphatidate phosphohydrolases in rat hepatocytes
- 13 April 1992
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 301 (1) , 103-106
- https://doi.org/10.1016/0014-5793(92)80219-7
Abstract
Incubation of hepatocytes with okadaic acid displaced the N-ethylmaleimide-sensitive phosphatidate phosphohydrolase from the membrane fraction into the cytosol and partially prevented the oleate-induced movement of phosphohydrolase from cytosol to membranes. However, higher concentrations of oleate still caused translocation and activation of the phosphohydrolase. This enzyme is stimulated by Mg2+, and is probably involved in glycerolipid synthesis. Okadaic acid also decreased the concentration of diacylglycerol within the hepatocytes. Okadaic acid had no observable effect on the activity of an N-ethylmaleimide-insensitive phosphatidate phosphohydrolase which remained firmly attached to membranes. This activity is not stimulated by Mg2+ and is probably involved in signal transduction by the phospholipase D pathway.Keywords
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