Anoxia reversibly suppresses neuronal calcium currents in rat hippocampal slices

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Abstract
Intracellular recording from CA1 neurons confirmed that short periods of anoxia (95% N2 + 5% CO2 for 2–4 min) have a hyperpolarizing action, caused by a rise in K conductance. After blockage of K channels with extracellular Cs+ and tetraethylammonium (or intracellular Cs+), large inward currents of Ca were evoked by depolarizing pulses: transient currents at a holding potential near −70 mV, and more sustained ones near −50 mV. Both types of Ca current were much reduced or fully suppressed after 1–3 min of anoxia, but they largely (or fully) recovered within 1–10 min of starting reoxygenation.