An in vitro Study of Fatty Acid Absorption.

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Abstract
An in vitro procedure was adapted for the study of fatty acid absorption in the hamster. C14-labeled palmitic acid when combined with bovine albumin and placed on the mucosal side of an intestinal sac preparation, enters the cell, is transformed into a glyceride, and subsequently is transfered to the serosal side. The resulting activity on the serosal side is 90% or more in the form of glycerides. The transfer of activity to the serosal side and incorporation of activity in the intestine is inhibited 20- and 16-fold, respectively, under anaerobic conditions. The relationships of the reported findings to fatty acid absorption is discussed.