Antibodies against basic fibroblast growth factor inhibit the autocrine growth of pulmonary artery endothelial cells
Open Access
- 6 June 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 233 (1) , 163-166
- https://doi.org/10.1016/0014-5793(88)81376-0
Abstract
Anti‐recombinant human basic fibroblast growth factor (rbFGF) antibodies efficiently inhibited the basal proliferation of bovine pulmonary artery endothelial (BAE) cells. The cell‐free extract of BAE cells stimulated the proliferation of bovine capillary endothelial cells and this activity was completely abolished by the antibodies. Furthermore, on heparin HPLC the activity was eluted at exactly the same retention time as that for authentic pituitary bFGF. These observations directly indicate that the BAE cells produce bFGF that stimulates their own basal growth by binding to specific receptors expressed on the cell surface.Keywords
This publication has 18 references indexed in Scilit:
- Expression of cDNA encoding human basic fibroblast growth factor in E.coliBiochemical and Biophysical Research Communications, 1987
- High and low affinity binding sites for basic fibroblast growth factor on cultured cells: Absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cellsJournal of Cellular Physiology, 1987
- Cloning and expression of cDNA encoding human basic fibroblast growth factorFEBS Letters, 1987
- Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growthNature, 1987
- Molecular and biological characterization of fibroblast growth factor, an angiogenic factor which also controls the proliferation and differentiation of mesoderm and neuroectoderm derived cellsCell Differentiation, 1986
- Identification of the fibroblast growth factor receptor of Swiss 3T3 cells and mouse skeletal muscle myoblastsBiochemistry, 1986
- Fibroblast growth factors: broad spectrum mitogens with potent angiogenic activityTrends in Biochemical Sciences, 1986
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Conjugation of antibodies with fluorochromes: Modifications to the standard methodsJournal of Immunological Methods, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970