A STUDY OF FIXATION FOR ELECTRON MICROSCOPY
Open Access
- 1 March 1952
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 95 (3) , 285-298
- https://doi.org/10.1084/jem.95.3.285
Abstract
Osmium tetroxide fixation of tissue blocks, as usually effected, is preceded by an acidification of the tissue. This acidification is probably responsible for morphological alterations which are notably disturbing in electron microscopy. The acidification and the resulting morphological alterations cannot be prevented by homogenizing the tissue directly in OsO4 solutions or by adding enzyme inhibitors (fluoride, iodoscetamide) to the fixative.Keywords
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