Neurochemical correlates of cyanide-induced hypoxic neuronal damage in vitro
- 1 February 1988
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 13 (2) , 159-163
- https://doi.org/10.1007/bf00973328
Abstract
Neuronal cortical cell cultures obtained from fetal mice were subjected to an hypoxic insult produced by sodium cyanide (1 mM) for 24 h. Neurochemical assays were performed 13–14 days after plating on intact cells in situ to determine if there was a specific pattern of cellular dysfunction in addition to morphologic change. Ro5-4864-displaceable benzodiazepine (BDZ) binding and high-affinity [3H]β-alanine uptake were not reduced when compared to control values. However, specific and clonazepam-displaceable BDZ binding (81±4% and 50±9% of control values, respectively), high-affinity [3H]GABA uptake (75±2%), and choline acetyltransferase activity (82±2%) were significantly lower. When the data were expressed in terms of protein content, high-affinity [3H]β-alanine uptake was significantlyincreased in cyanide-exposed and magnesium-treated cultures (123±5% and 117±3%, respectively) as was R05-4864-displaceable BDZ binding (152±14%), consistent with stimulation of nonneuronal BDZ binding and increased glial neurotransmitter uptake. Moreover, pretreatment of the cultures with magnesium effectively prevented both the morphologic and neurochemical evidence of hypoxic injury. These data lend further support to the notion that the release of excitatory neurotransmitters may mediate neurotoxicity in developing brain.This publication has 34 references indexed in Scilit:
- Barbiturate protection against hypoxic neuronal damage in vitroJournal of Neurosurgery, 1986
- Autoradiographic Localization of Benzodiazepine Receptor Binding in Dissociated Cultures of Fetal Mouse Cerebral CortexJournal of Neurochemistry, 1986
- Glutamate neurotoxicity in cortical cell culture is calcium dependentNeuroscience Letters, 1985
- New Insights into Mechanisms of Neuronal Damage in the Developing BrainPediatric Neurosurgery, 1985
- Elevation of the Extracellular Concentrations of Glutamate and Aspartate in Rat Hippocampus During Transient Cerebral Ischemia Monitored by Intracerebral MicrodialysisJournal of Neurochemistry, 1984
- Focal Cortical Seizures Cause Distant Thalamic LesionsScience, 1982
- A method for large-scale production of mouse brain cortical culturesDevelopmental Brain Research, 1982
- Benzodiazepine Receptor Development in Cultures of Fetal Mouse Cerebral Cortex Mimics Its Development in vivoDevelopmental Neuroscience, 1982
- Cell Damage in the Brain: A Speculative SynthesisJournal of Cerebral Blood Flow & Metabolism, 1981
- Features of Astrocytic Function Apparently Involved in the Response of Central Nervous Tissue to Ischemia-HypoxiaJournal of Cerebral Blood Flow & Metabolism, 1981