Human monoclonal IgM with autoantibody activity against two gangliosides (GM1 and GDlb) in a patient with motor neuron syndrome

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Abstract
SUMMARY: Small amounts of oligoclonal immunoglobulins were detected by Western blotting in the serum from a patient with motor neuron syndrome. The prominent one. a monoclonal IgMλ, reacted strongly with the gangliosides GM1 and GDlb and more weakly with asialo GM1, as shown by immunoenzymatic staining of thin-layer chromatograms of gangliosides, ELISA on purified glycolipid coats and immunoadsorption with purified GM1. Affinity-chromatography with purified GMI resulted in the purification of monoclonal IgMλ. This purified IgM and its Fab fragments showed the same pattern of reactivity with gangliosides as that observed with whole serum. Such monoclonal IgM could be responsible for motor neuron diseases in some patients with overt or barely detectable monoclonal gammopathies.