Intracellular pH, H ion flux and H ion permeability coefficient in bullfrog toe muscle

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Abstract
1. The dimethyloxazolidinedione (DMO) technique was used to estimate intracellular pH (pHi) in bullfrog toe muscles incubated in vitro. The control value of pHi was 7·16 + ± 0·01 ( S.D.).2. pHi was affected by changes in PCO2 and external bicarbonate ion concentration ([HCO3−]0). At a given PCO2, decreasing the external [HCO3−] was more effective in lowering pHi than increasing the external [HCO3−] was in increasing pHi.3. On the assumption that the changes in pHi were due to hydrogen ion [H+) movements across the membrane, a H+ flux of 10−13 mole/cm2. sec was calculated. The corresponding H+ permeability coefficient was 10−3 cm/sec.4. The variability of the tissue CO2 buffer value was examined.