Use of a Low‐Ionic‐Strength Medium in Manual Tests for Antibody Detection

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Abstract
The present observations confirm the value of suspending [human] red blood cells in a low-ionic-strength medium in the 1st stage of the indirect antiglobulin test, i.e., during the period of incubation with antibody-containing serum. The main advantage of this procedure is to shorten the time of incubation. In this respect, a low-ionic-strength medium appears to be superior to albumin as a suspending medium for the red blood cells. A further advantage is to increase the uptake of certain antibodies; this effect was pronounced with selected Rh antibodies believed to be of low affinity.