Abstract
A simple aerobic and anaerobic technique, with a medium containing low peptone conen. and relatively high carbohydrate concn., distinguishes the basic difference between oxidative and fermentative metabolism of carbohydrates. 3 taxonomically significant groups of bacteria[long dash](I) nonoxidizers, nonfermenters, (II) oxidizers, nonfermenters, (HI) fermenters[long dash]are readily separated on the basis of differences in these metabolic patterns. Alcaligenes faecalis is associated with group I, Pseudomonas aeruginosa, Bacterium anitratum, Agrobacterium tumefaciens and Malleomyces pseudomallei with group II, and Enterobacteriaceae, Vibrio comma and Aeromonas liquefaciens with group III.