Evaluation of Three Current Methods for the Determination of Creatine Kinase-MB Catalytic Activity

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Abstract
Three current methods for the determination of the creatine kinase MB activity were evaluated: electrophoresis (Beckman Inst.), ion-exchange MB-Zyme (J. T. Baker) and ion-exchange on aca (Du Pont). Patients (80) were selected out of 3 groups: acute myocardial infarction, heart and vascular surgery patients and a number of noncardiac cases. The precision of the ion-exchange methods was comparable; CV [coefficient of variation] 5.7% (aca) and CV 5.2% (MB-Zyme) for a high creatine kinase MB pool. Electrophoresis overestimated the creatine kinase MB activity and was less precise (CV 9.6%) and less sensitive than the ion-exchange methods. The aca (Du Pont) method was less specific than the MB-Zyme. The main source of discrepancy was samples from certain patients in the surgery unit, which gave falsely high values by the aca method, and showed altered mobility in electrophoresis, suggesting an atypical creatine kinase. Three macro creatine kinase type I included in this study were erroneously determined as creatine kinase MB by both the ion-exchange methods.