In situ hybridization of an acetylaminofluorene-modified probe recognized by Z-DNA antibodies in vitro
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 42 (1-2) , 105-109
- https://doi.org/10.1159/000132260
Abstract
An in situ hybridization procedure, based on the chemical modification of DNA by acetylamino-fluorene (AAF), followed by a specific immunoreaction was used to localize a Z-DNA sequence isolated from the satellite DNA of Cebus appella. The AAF probe is localized on the R-band-positive heterochromatic segments of Cebus chromosomes, which strongly react with Z-DNA antibodies. The use of a nonradioactive single-stranded labeled probe confirms the reliability and the rapidity of immunochemical methods for the detection of DNA sequences on chromosomes.Keywords
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