Isolation and chemical conversion of two novel prostaglandin endoperoxides: 5(6)-epoxy-PGG1and 5(6)-epoxy-PGH1
- 9 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 172 (2) , 279-283
- https://doi.org/10.1016/0014-5793(84)81141-2
Abstract
5(6)-Epoxy-PGG1 and 5(6)-epoxy-PGH1 were isolated after incubation of microsomes of RSV with 3H-labelled 5(6)-epoxy-C20:3 for 45 s at 37°C. The endoperoxides were methylated and characterized by conversion to prostaglandins. In buffer, the endoperoxides were converted to methyl-5(6)-epoxy-PGE1 and methyl-5(6)-epoxy-15-hydroperoxy-PGE1, while treatment with SnCl2 reduced the endoperoxides to methyl-5-hydroxy-PGI1α and methyl-5-hydroxy-PGI1β. Significant amounts of methyl-5(6)-epoxy-HHD were also formed. The endoperoxides could be separated by silicic acid chromatography and when 1 mM phenol was present in the incubation, 5(6)-epoxy-PGH1 was obtained as the main product.Keywords
This publication has 12 references indexed in Scilit:
- Epoxyeicosatrienoic acids stimulate glucagon and insulin release from isolated rat pancreatic isletsBiochemical and Biophysical Research Communications, 1983
- NOVEL HYPOTHALAMIC ARACHIDONATE PRODUCTS STIMULATE SOMATOSTATIN RELEASE FROM THE MEDIAN EMINENCE1Endocrinology, 1983
- Metabolism of arachidonic acid by isolated rat hepatocytes, renal cells and by some rabbit tissuesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1982
- Novel epoxides formed during the liver cytochrome P-450 oxidation of arachidonic acidBiochemical and Biophysical Research Communications, 1982
- Chemical and enzymic syntheses of 5-HPETE, a key biological precursor of slow-reacting substance of anaphylaxis (SRS), and 5-HETEJournal of the American Chemical Society, 1980
- Some biological effects of prostaglandin endoperoxide analogsLife Sciences, 1976
- Prostaglandin Endoperoxides. Novel Transformations of Arachidonic Acid in Human PlateletsProceedings of the National Academy of Sciences, 1974
- Isolation and Structure of Two Prostaglandin Endoperoxides That Cause Platelet AggregationProceedings of the National Academy of Sciences, 1974
- Isolation and properties of intermediates in prostaglandin biosynthesisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Detection and Isolation of an Endoperoxide Intermediate in Prostaglandin BiosynthesisProceedings of the National Academy of Sciences, 1973