Identification of hemolytic activity in Prevotella intermedia
- 1 April 1998
- journal article
- research article
- Published by Wiley in Oral Microbiology and Immunology
- Vol. 13 (2) , 97-105
- https://doi.org/10.1111/j.1399-302x.1998.tb00719.x
Abstract
Hemolysin production was measured in strains of Prevotella intermedia. Zones of beta-hemolysis were detected on agar plates supplemented with either sheep, rabbit or human erythrocytes. A standard tube assay was performed on cell suspensions of the organism to measure hemolytic activity, which was found to be dose dependent, eliminated by heat treatment, and saturable with increasing concentrations of blood. Growth-phase experiments suggested that hemolysin production was increased during logarithmic growth and was reduced during stationary phase. Cell fractionation, performed on several strains of P. intermedia, localized the activity in the outer membrane and in cell vesicles. The biological implication of this study is that P. intermedia, by virtue of its hemolytic activity, is capable of liberating the hemoglobin from erythrocytes, thereby acquiring an essential nutrient, iron, for its metabolism.Keywords
This publication has 61 references indexed in Scilit:
- Bacteriologic Findings in Peritonsillar Abscesses in Young AdultsClinical Infectious Diseases, 1993
- Peritonsillar Abscess, Retropharyngeal Abscess, Mediastinitis, and Nonclostridial Anaerobic Myonecrosis: A Case ReportClinical Infectious Diseases, 1993
- Characterization of hemolytic bacteria in subgingival plaqueJournal of Periodontal Research, 1993
- Haemagglutinating and haemolytic activity of the extracellular vesicles of Bacteroides gingivalis W50Oral Microbiology and Immunology, 1990
- Occurrence of Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in severe periodontitis in relation to age and treatment historyJournal of Clinical Periodontology, 1990
- The distribution of trypsin‐like enzyme activity in cultures of a virulent and an a virulent strain of Bacteroides gingivalis W50Oral Microbiology and Immunology, 1989
- Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseasesJournal of Clinical Periodontology, 1988
- Phospholipase A activity of extracellular products from Bacteroides melaninogenicus on epithelium tissue culturesJournal of Periodontal Research, 1985
- Cytotoxic Activity of Partially Purified Escherichia Coli Alpha HaemolysinJournal of Medical Microbiology, 1982
- Phospholipase A activity in supernatants from cultures of Bacteroides melaninogenicusBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1981