Properties of Phage-receptor Lipopolysaccharide from Pseudomonas morsprunorum

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Abstract
Lipopolysaccharide (LPS) of P. morsprunorum was extracted with hot phenol and purified by repeated centrifuging followed by block electrophoresis or gel filtration. LPS from a virulent isolate exhibited specific phage [A 7] inactivation (PI50 [50% decrease in plaque formation] = 0.05 .mu.g LPS ml-1); LPS from an avirulent phage-resistant mutant did not. LPS was considered pure when a single band was detected following sodium dodecyl sulfate-cellulose acetate electrophoresis (pH 7.4). It was not phytotoxic when inoculated into cherry leaves at concentrations up to 1 mg ml-1, but produced weak chlorosis in bean and tobacco at 2 mg ml-1; no visible symptoms appeared after treatment with low concentrations. The chemical composition of the LPS was partly determined.