Intranuclear changes in the polyhedrosis of Tipula paludosa (Meig.)

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Abstract
By means of ultra-thin sections for electron microscopy it has been possible to observe intra-nuclear changes in the polyhedrosis of larvae of Tipula paludosa. The virus rods arise in the centres of the greatly enlarged nuclei of blood cells. Thin-walled vesicles, apparently filled with fluid, form round individual virus rods and collect in masses on the inner wall of the nuclear membrane. Crystallization then begins, starting at the edge against the nuclear membrane. The vesicles are gradually compressed round the rods, eventually forming a capsule. Sections through free virus particles show them to be enclosed in a membrane, a second inner membrane round the substance of the rod may also be present.There appears to be no capsule round the free virus particles such as encloses the virus particles inside the polyhedral crystal.