Radio-Isotopic Determination of Subnanomolar Amounts of Peroxide
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 5 (6) , 359-368
- https://doi.org/10.3109/10715768909073419
Abstract
A new radio-isotopic method for determination of peroxide in subnanomolar concentrations has been described. The method is based on the reactivity of peroxide with alpha-Ketoglutaric acid containing [I-14C]-alpha-Ketoglutaric acid, and measurement of the resulting 14CO2 by radioactivity. The recovery of standard peroxide, alone or mixed with some biologically derived samples, following this technique was found to be 97 +/- 2.7%. The method may therefore be useful for determination of peroxides in active biological samples.Keywords
This publication has 3 references indexed in Scilit:
- Peroxide Damage to the Eye LensIn VitroPrevention by PyruvateFree Radical Research Communications, 1988
- THE RESPIRATION MECHANISM OF PNEUMOCOCCUS. IIIThe Journal of Experimental Medicine, 1934
- Notice sur l'action de l'eau oxygénée sur les acides α‐cétoniques et sur les dicétones 1. 2Recueil des Travaux Chimiques des Pays-Bas et de la Belgique, 1904