Apparatus for study of ventricular function and metabolism in the isolated perfused rat heart.

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Abstract
The use of oxygenated buffer and constant coronary flow instead of constant pressure to perfuse the isolated rat heart was described. An indwelling balloon containing a known volume of fluid was employed to record intra-cavitary pressure during osovolumic paced contractions. This technic resulted in adequate oxygenation, as evidenced by lactate extraction instead of production, and provided a steady nonfailing preparation. When it is perfused at 9-10 ml/min., paced at 220-240 beats/min., and maintained at a temperature of 37 degrees C, the rat heart generates a stable isovolumic systolic pressure of 80-120 mm Hg for several hr.

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