RELATION OF HEMOGLOBIN TO THE RED CELL MEMBRANE*†

Open Access
Publisher Website
Google Scholar
Abstract
The problem of the relation of hemoglobin to the red cell membrane is reviewed. A method is described for preparing membranes and it is shown that they contain reproducible amounts of hemoglobin, N, P and total solids. Denaturation and fragmentation of membranes occur readily with variation of the experimental conditions in any stage of the processing and the anticoagulant used is of particular importance. A simple direct method for measuring hemoglobin attached to the insoluble membranes is described. The amount of hemoglobin found with the membrane is so regular and so large (1 50% of dry weight) as to indicate that the 2 are somehow bound.