Conformational heterogeneity in polypeptide cardiac stimulants from sea anemones
- 20 August 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 174 (1) , 15-19
- https://doi.org/10.1016/0014-5793(84)81068-6
Abstract
High-resolution 1H NMR spectra at 300 MHz of the polypeptide cardiac stimulants anthopIeurin-A and Anemonia sulcata toxin II reveal conformational heterogeneity in both molecules. The two conformations, manifest in a number of split 1H resonances, are in slow exchange over a wide range of pH and temperature. Heterogeneity affects a region of these molecules containing the structurally and functionally important Asp residues. By comparison with a homologous polypeptide Anemonia sulcata toxin I, which does not show this type of heterogeneity, it is suggested that the heterogeneity may originate in cis-trans isomerism of the Gly-40 to Pro-41 peptide bond.Keywords
This publication has 16 references indexed in Scilit:
- Natural-Abundance 13C Nuclear-Magnetic-Resonance Study of Toxin II from Anemonia sulcataEuropean Journal of Biochemistry, 2005
- Proton nuclear magnetic resonance spectroscopic study of the polypeptide toxin I from Anemonia sulcataBiochemistry, 1984
- Nuclear magnetic resonance study of interaction of ligands with Streptococcus faecium dihydrofolate reductase labeled with [.gamma.-13C]tryptophanBiochemistry, 1982
- Interaction of the polypeptide cardiac stimulant Anthopleurin-A with H+, Ca2+, and membrane lipidsArchives of Biochemistry and Biophysics, 1982
- ELECTROMECHANICAL EFFECTS OF ANTHOPLEURIN‐A (AP‐A) ON RABBIT VENTRICULAR MUSCLE: INFLUENCE OF DRIVING FREQUENCY, CALCIUM ANTAGONISTS, TETRODOTOXIN, LIDOCAINE AND RYANODINEBritish Journal of Pharmacology, 1981
- Purification and pharmacological properties of eight sea anemone toxins from Anemonia sulcata, Anthopleura xanthogrammica, Stoichactis giganteus and Actinodendron plumosumBiochemistry, 1981
- Systematic Application of Two‐Dimensional 1H Nuclear‐Magnetic‐Resonance Techniques for Studies of ProteinsEuropean Journal of Biochemistry, 1981
- Structural properties of toxin II of sea anemone (Anemone sulcata) determined by laser raman spectroscopyFEBS Letters, 1976
- Isolation and characterisation of three polypeptides with neurotoxic activity from Anemonia sulcataFEBS Letters, 1975
- Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfateAnalytical Biochemistry, 1971