OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought‐, high‐salt‐ and cold‐responsive gene expression
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Open Access
- 28 February 2003
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 33 (4) , 751-763
- https://doi.org/10.1046/j.1365-313x.2003.01661.x
Abstract
Summary: The transcription factors DREBs/CBFs specifically interact with the dehydration‐responsive element/C‐repeat (DRE/CRT) cis‐acting element (core motif: G/ACCGAC) and control the expression of many stress‐inducible genes in Arabidopsis. In rice, we isolated five cDNAs for DREB homologs: OsDREB1A, OsDREB1B, OsDREB1C, OsDREB1D, and OsDREB2A. Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high‐salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over‐expression of OsDREB1A in transgenic Arabidopsis induced over‐expression of target stress‐inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high‐salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress‐inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over‐expressed in the OsDREB1A transgenic Arabidopsis. The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1‐type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high‐salt, and/or cold stresses.Keywords
This publication has 39 references indexed in Scilit:
- DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene ExpressionBiochemical and Biophysical Research Communications, 2002
- ORCA3, a Jasmonate-Responsive Transcriptional Regulator of Plant Primary and Secondary MetabolismScience, 2000
- Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathwaysCurrent Opinion in Plant Biology, 2000
- Arabidopsis Ethylene-Responsive Element Binding Factors Act as Transcriptional Activators or Repressors of GCC Box–Mediated Gene ExpressionPlant Cell, 2000
- AnArabidopsisGene Family Encoding DRE/CRT Binding Proteins Involved in Low-Temperature-Responsive Gene ExpressionBiochemical and Biophysical Research Communications, 1998
- THE MOLECULAR BASIS OF DEHYDRATION TOLERANCE IN PLANTSAnnual Review of Plant Biology, 1996
- A Dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2.Plant Cell, 1996
- AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth.Plant Cell, 1996
- Efficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous PlantsPlant and Cell Physiology, 1996
- A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress.Plant Cell, 1994