Microinjection of 14C-sucrose and other tracers into isolated phloem strands of Heracleum

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Abstract
Microinjection of solutions of 14C-sucrose, 14C-aspartic acid, K332PO4, 42KCl, and 3HHO have all been made into living sieve elements of displaced, isolated, phloem strands of Heracleum. The profile of tracer movement is reported in experiments between 1 min 40 s and 8 min in duration in strands prepared both with and without callose-reducing buffer under conditions where lateral transfer of tracer is restricted. Two types of translocation patterns are discernable, a mass flow near the origin and a type of pulse flow superimposed on it and extending along the strands in both directions simultaneously. Some 42K and 32P has been detected with the 14C in the pulses, but 14C-sucrose also appears to move independently of the other tracers.

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