ISOZYME PROFILES OF OVAL CELLS, PARENCHYMAL-CELLS, AND BILIARY CELLS ISOLATED BY CENTRIFUGAL ELUTRIATION FROM NORMAL AND PRENEOPLASTIC LIVERS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (1) , 332-338
Abstract
The fetal liver isozymes aldolase A and pyruvate kinase K increase in livers of adult rats fed a choline deficient-diet containing 0.1% ethionine. Oval cells isolated by centrifugal elutriation from preneoplastic livers of animals receiving the carcinogenic diet contained these fetal forms as well as fetal-adult isozyme hybrids. Parenchymal cells isolated from the livers of these animals had only aldolase B and pyruvate kinase L, the same isozymes present in parenchymal cells of normal adult rats. Liver homogenates from rats receiving the carcinogenic diet contain lactate dehydrogenase (LDH) 1, LDH 2 and LDH 3 in addition to LDH 4 and LDH 5, which are the forms detected in normal liver homogenates. LDH 1, LDH 2 and LDH 3 are present in oval cells of preneoplastic livers and in biliary epithelial cells of normal livers, but not in parenchymal cells isolated from normal and preneoplastic livers. Cells of biliary epithelium from normal livers also contain aldolase A and pyruvate kinase K, but not the fetal-adult isozymes present in oval cell populations. In animals receiving this carcinogenic diet, isozyme alterations associated with neoplasia result from the proliferation of a new cell population which contains these enzymes and not from dedifferentiation of mature hepatocytes. This new cell population may include a liver stem cell compartment containing cells in transitional states of differentiation.