Large-scale isolation of human erythrocyte Ca2+-transport ATPase
- 1 October 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 207 (1) , 155-159
- https://doi.org/10.1042/bj2070155
Abstract
A rapid procedure for preparing large quantities of purified erythrocyte Ca2+-transport ATPase is presented. The method involves: (1) fast preparation of calmodulin-deficient, essentially haemoglobin-free, erythrocyte membranes by molecular filtration using Pellicon filters; (2) solubilization of membrane proteins by deoxycholate; and (3) a batch procedure using calmodulin-Sepharose 4B gel for purification of Ca2+-transport ATPase.This publication has 14 references indexed in Scilit:
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