Separation of Parent Benzodiazepines and Their Major Metabolites by Reverse-Phase Ion-Pair Chromatography
- 1 November 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 6 (6) , 286-289
- https://doi.org/10.1093/jat/6.6.286
Abstract
A novel reverse-phase-ion-pair (RPIP), isocratic, high performance liquid chromatographic (HPLC) separation technique is presented for the analysis of all current parent benzodiazepines and their major pharmacologically active metabolites utilizing variable ultraviolet (UV) detection. In this RPIP technique, the counter-ion, methanesulfonic acid, forms an ion-pair or associaiton-complex with the ionic forms of the benzodiazepines, which are then retained by the reverse-phase C8 column.This publication has 13 references indexed in Scilit:
- MONITORING CLORAZEPATE DIPOTASSIUM AS DESMETHYLDIAZEPAM IN PLASMA BY ELECTRON-CAPTURE GAS-LIQUID-CHROMATOGRAPHY1980
- Monitoring diazepam and desmethyldiazepam concentrations in plasma by gas-liquid chromatography, with use of a nitrogen-sensitive detector.Clinical Chemistry, 1979
- Simultaneous gas-chromatographic analysis for diazepam and its major metabolite, desmethyldiazepam, with use of double internal standardization.Clinical Chemistry, 1978
- Analysis of lorazepam and its glucoronide metabolite by electron-capture gas—liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1978
- Determination of Desmethyldiazepam in Plasma by Electron-Capture GLC: Application to Pharmacokinetic Studies of ClorazepateJournal of Pharmaceutical Sciences, 1978
- Application of Reverse Phase Ion-Pair Partition Chromatography to Drugs of Forensic InterestJournal of AOAC INTERNATIONAL, 1977
- Determination of flunitrazepam in body fluids by means of high-performance liquid chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1977
- The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high-performance liquid chromatographyJournal of Pharmacy and Pharmacology, 1977
- Determination of clorazepate and its major metabolites in blood and urine by electron capture gas-liquid chromatographyJournal of Chromatography A, 1977
- Quantitative determination of pinazepan and its metabolites in blood and urine by high-performance liquid chromatographyJournal of Chromatography A, 1977