Isolation of cDNA and genomic DNA clones encoding type II collagen
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (10) , 4207-4228
- https://doi.org/10.1093/nar/12.10.4207
Abstract
A cDNA library constructed from total chick embryo RNA was screened with an enriched fraction of type II collagen mRNA. Two overlapping cDNA clones were characterized and shown to encode the COOH propeptide of type II collagen. In addition, a type II collagen clone was isolated from a Charon 4A library of chick genomic fragments. Definitive identification of the clones was based on DNA sequence analysis. The 3′ end of the type II collagen gene appears to be similar to that of other interstitial collagen genes. Northern hybridization data indicates that there is a marked decrease in type II collagen mRNA levels in chondrocytes treated with the dedifferentiating agent 5-bromodeoxyuridine. The major type II collagen mRNA species is 5300 bases long, similar to that of other interstitial collagen RNAs.Keywords
This publication has 36 references indexed in Scilit:
- Levels of collagen mRNA in dedifferentiating chondrocytesExperimental Cell Research, 1982
- Regulation of the synthesis of extracellular matrix components in chondroblasts transformed by a temperature-sensitive mutant of Rous sarcoma virusCell, 1982
- Structure of the pro α2(I) collagen geneNature, 1981
- Regulation of procollagen messenger ribonucleic acid levels in Rous sarcoma virus transformed chick embryo fibroblastsBiochemistry, 1981
- Sequence determination and analysis of the 3' region of chicken Pro-.alpha.1(I) and Pro-.alpha.2(I) collagen messenger ribonucleic acids including the carboxy-terminal propeptide sequencesBiochemistry, 1981
- Identification of a Balb/c Mouse Proα1(I) Procollagen Gene: Evidence for Insertions or Deletions in Gene Coding SequencesDNA, 1981
- The collagen gene: Evidence for its evolutionary assembly by amplification of a DNA segment containing an exon of 54 bpCell, 1980
- Isolation of the chicken β-globin gene and a linked embryonic β-like globin gene from a chicken DNA recombinant libraryCell, 1979
- Regulation of casein messenger RNA during the development of the rat mammary glandBiochemistry, 1975
- Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsinBiochemistry, 1972