ANALYSIS OF DIFFERENTIATION OF MOUSE HEMATOPOIETIC STEM-CELLS IN CULTURE BY SEQUENTIAL REPLATING OF PAIRED PROGENITORS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 64  (2) , 393-399
Abstract
Blast cell colonies seen in cultures of spleen cells from 5-fluorouracil-treated mice provide a highly enriched population of primitive hemopoietic progenitors. Recent studies of the differentiation potentials of the paired daughter cells of these progenitors showed different patterns of differentiation in the colonies produced by the separated daughter cells. Sequential micromanipulation of paired progenitors was carried out followed by cytologic examinations of the colonies derived from these progenitors. Of the total 94 evaluable cultures, consisting of 3 or more colonies, 52 consisted of macrophage colonies and 1 consisted of megakaryocyte colonies. In the remaining 41 cultures, diverse combinations of colonies revealing heterogeneous compositions of cell lineages were identified. Presumptive genealogic trees of the differentiation of hemopoietic progenitors constructed for the latter group of cultures suggested that monopotent progenitors may be derived from pluripotent progenitors in 2 ways; directly during 1 cell division of pluripotent cells or as a result of progressive lineage restriction during successive division of the pluripotent progenitors. Some of the oligopotent progenitors apparently are capable of limited self-renewal.