Apolipoprotein B–Gene DNA Polymorphisms Associated with Myocardial Infarction

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Abstract
Levels of apolipoprotein B, the protein component of low-density lipoproteins, correlate with the risk of coronary heart disease. We examined whether genetic variation in apolipoprotein B is associated with myocardial infarction by studying apolipoprotein B–gene restriction-fragment–length polymorphisms in 84 patients with myocardial infarction and an equal number of matched controls. Southern blot analysis with apolipoprotein B–gene probes, performed after DNA was digested with the endonucleases Xbal and EcoRI, revealed alleles that we designated as X1, X2, and X3 and as R1 and R2, respectively. Similar studies with the endonuclease Mspl revealed alleles of many different sizes (the difference was due to an insertion–deletion polymorphism), which we grouped as larger and smaller alleles and designated as ID1 and ID2, respectively.