The permeability of rat liver lysosomes to sugars. Evidence for carrier-mediated facilitated diffusion

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Abstract
By the osmotic-protection method, the penetration of sugars through the rat liver lysosomal membrane was studied to determine whether sugar uptake was by facilitated diffusion. The following criteria for this type of transport were established: sugar specificity, the order of uptake being 2-deoxy-D-glucose < D-glucose < D-mannose < D-galactose < D-ribose < 2-deoxy-D-ribose; stereospecificity, the uptake of L-glucose and L-ribose being 50% slower than their D-stereoisomers; inhibition by 1 mM-phlorrhizin and 1 M-cytochalasin B; competition between sugars for uptake and a Q10 (rate difference over a 10.degree. C temperature range) for uptake of approximately 2.8. Apparently, sugar uptake into lysosomes from rat liver is by facilitated diffusion.