Development and Evaluation of a New Commercial Test Allowing the Simultaneous Detection of Noroviruses and Sapoviruses by Reverse Transcription-PCR and Microplate Hybridization

Publisher Website
Google Scholar
Abstract
This work describes the design and initial evaluation of a commercial test allowing the detection of noroviruses and sapoviruses by reverse transcription-PCR (RT-PCR) in a single tube followed by microplate hybridization, as well as the detection of PCR inhibitors. The test was shown to be broadly reactive (except for Melksham-like strains), sensitive, and specific and thus should be useful for calicivirus detection in clinical practice.