Monoclonal antibody to spleen focus-forming virus-encoded gp52 provides a probe for the amino-terminal region of retroviral envelope proteins that confers dual tropism and xenotropism

Publisher Website
Google Scholar
Abstract
Monoclonal antibodies which recognize a region common to Friend spleen focus-forming virus encoded gp52 and Friend mink cell focus-inducing viral gp70 were isolated. One such antibody from hybridoma 7C10 was tested extensively in immune precipitation and was found to react with a determinant on envelope gp70 of all mink cell focus-inducing, xenotropic and amphotropic mouse retroviruses tested, but not with envelope gp70 of ecotropic viruses, including Friend, Moloney and AKR murine leukemia viruses. Monoclonal antibody from hybridoma 7C10 precipitated a 23,000 MW fragment, derived by V8 protease digestion of Friend mink cell focus-inducing gp70. This 23,000 MW peptide was determined to derive from the amino terminus of the molecule. Thus, endogenously acquired sequences of mink cell focus-inducing viruses are found at the 5'' end of the envelope gene.

This publication has 45 references indexed in Scilit: