Ovine Corticotropin-Releasing Factor Stimulates Somatostatin Secretion from Cultured Brain Cells*

Abstract
Ovine corticotropin-releasing factor (CRF) and sauvagine stimulated the secretion of somatostatin-like immunoactivity from dispersed cells of fetal rat brain maintained in long term cultures. Deamidated CRF and CRF-(10–41) were inactive. CRF-stimulated secretion was blocked by cobalt in a low calcium buffer and by tetrodotoxin. The data are consistent with an extracellular regulatory role for CRF within the central nervous system.

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