Hemocyanins in Spiders, XX. Sulfhydryl Groups and Disulfide Bridges in SubunitdofEurypelma californicumHemocyanin
- 1 January 1985
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 366 (1) , 403-410
- https://doi.org/10.1515/bchm3.1985.366.1.403
Abstract
Subunit d of E. californicum hemocyanin contains, after reduction, 7 cysteine residues. Using 3,3''-dithiobis(6-nitrobenzoic acid), 3 mol cysteine/mol subunit were determined. The cysteine- and cystine-containing peptides of subunit d were obtained by cyanogen bromide cleavage and subsequent treatment with trypsin. The free cysteines were established at positions 102, 261 and 454, respectively. Cys205-Cys210 and Cys529-Cys579 are connected by disulfide bridges.This publication has 11 references indexed in Scilit:
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