REVERSAL OF ISCHEMIC DAMAGE WITH AMINO-ACID SUBSTRATE ENHANCEMENT DURING REPERFUSION

  • 1 January 1980
    • journal article
    • research article
    • Vol. 88  (5) , 702-709
Abstract
Dogs (20) underwent 15 min of normothermic ischemic arrest and 30 min of reperfusion while on cardiopulmonary bypass. In 10 control dogs, the reperfusate blood was not modified. In 10 other dogs the aorta was reclamped and the heart reperfused for 5 min with blood containing L-glutamate (0.026M). Coronary blood flow (microspheres), left ventricular (LV) metabolism [O2 content, ATP], LV compliance (intraventricular balloon) and LV performance (balloon and Starling curves) were measured before and 30 min after ischemia. Ischemic arrest for 15 min produced significant depression in contractility and oxidative metabolism. L-Glutamate infusion resulted in higher O2 uptakes and allowed more complete recovery of ATP content. Glutamate-treated hearts had more complete recovery in the rate of contraction (+dP/dt) and relaxation (-dP/dt), the best recovery of compliance and complete recovery of stroke work index. The addition of L-glutamate to reperfusate blood reverses ischemic damage. L-glutamate acts by replenishing Krebs'' cycle intermediates lost during ischemia, stimulating oxidative metabolism and enhancing ATP production.

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