Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, Forskolin
- 1 January 1994
- journal article
- Published by Springer Nature in Cytotechnology
- Vol. 16 (2) , 101-108
- https://doi.org/10.1007/bf00754612
Abstract
The ratio of hapten to bovine serum albumin in an antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibodies against forskolin was produced by fusing splenocytes immunized with a forskolin-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cells. The cross-reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.57%. A very small cross-reaction appeared with 1-deoxy, 9-deoxy and 1,9-dideoxy forskolin derivatives. The full measuring range of the assay extends from 6 ng to 200 ng of forskolin. The competitive ELISA assay used for this analysis was found to be more sensitive than TLC (10 μg), GLC (30 ng) and HPLC (1 μg) methods.Keywords
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