An analysis of seed development inPisum sativumXVIII. The isolation of mutants defective in embryo development

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Abstract
Following EMS mutagenesis of pea (Pisum sativum L.) seed, nine mutants have been isolated whose embryos are affected in their development, being defective either in organogenesis or in cell differentiation. Studies of their morphology at the whole organ level and their histology indicated at least four groups: those with single cotyledons (representing at least three loci), two blocked at the early cotyledonary stage, one with abnormal root development and two with cellular defects. Genetic analysis indicated the mutants were behaving as monogenic recessive mutations. These mutants represent the first of their type for legumes and should prove especially useful, therefore, for examining those aspects of development characteristic of these species.

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