Fluorescence in Paraffin Sections of Dye-Labelled Protein Administeredin vivo:Effect of HgCl2Fixation
- 1 January 1968
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 43 (2) , 59-61
- https://doi.org/10.3109/10520296809115043
Abstract
Lung and liver slices, 2-3 mm thick, from guinea pigs injected intravenously with fluorescent dye-protein conjugate are fixed for 15-30 min. in saturated aqueous HgCl2, dehydrated in ethanol, cleared in xylene and embedded in paraffin at 60 C. Mercurial deposits are removed with I2KI from 5 [mu] sections taken to water, and the iodine then removed with 5% Na2S2O3. Sections are mounted from xylene into permanent nonfluorescent mounting medium. This procedure gives optimal fluorescence which is not decreased by the technic of removing mercurial precipitates. Longer fixation, fixation in phosphate-buffered formalin, or in an HgCl2-forma-lin mixture given inferior results.This publication has 1 reference indexed in Scilit:
- FLUORESCENT PROTEIN TRACERS A SIMPLE ALTERNATIVE TO FLUORESCEINThe Lancet, 1958