The Application of Acetolysis for Releasing Leaf Cuticular Membranes ofPandanusin Taxonomic Studies
- 1 January 1971
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 46 (5) , 227-232
- https://doi.org/10.3109/10520297109067862
Abstract
Leaf cuticular membranes can be released cleanly and in one piece after a 10 min treatment of leaf in a 100 C acetolysis mixture: acetic anhydride, 9; and concentrated H2SO4,1 v/v. This method has been used till now only for studying pollen and spore morphology but was found quite appropriate for a taxonomic study of the leaf surface of the genus Pandanus.Keywords
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