EFFECTS OF NEURAMINIDASE AND GALACTOSE OXIDASE ON MURINE LYMPHOCYTES .1. EVIDENCE FOR DIFFERENTIAL DELIVERY OF SIGNAL(S) LEADING TO CELL-PROLIFERATION AND DIFFERENTIATION OF CYTOTOXIC T CELLS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 119  (6) , 2163-2171
Abstract
The sequential treatment of normal C57BL/6 mouse spleen cell populations with neuraminidase (NA) and galactose oxidase (GO) resulted in cell proliferation, but not in the differentiation of cytotoxic T [thymus-derived] cells. C57BL/6 spleen cells derived from animals primed 5-8 mo. earlier with alloantigen (P815 mastocytoma cells of the DBA/2 strain) proliferated and demonstrated T cell-mediated cytotoxicity after NAGO stimulation. T cells differentiating into cytotoxic cells after NAGO treatment demonstrated properties similar to alloantigen-specific memory T cells. These were as follows: cytotoxicity developed only from primed cell populations; cytotoxicity developed within 24 h after NAGO treatment; DNA synthesis was not required for the differentiation of cytotoxic cells during the first 24 h of culture but both DNA synthesis and cell proliferation were required for the cytotoxicity developing after 24 h; all cytotoxicity induced by NAGO showed specificity for the priming alloantigen. Cytotoxicity could be induced at much lower GO concentrations than needed for increased DNA synthesis. NAGO can apparently differentially deliver 2 signals to T lymphocytes: 1 leading to cell proliferation, the other causing the differentiation of memory T cells into cytotoxic effectors.

This publication has 1 reference indexed in Scilit: