On the Nature of DNA Promoter Conformations

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Abstract
Low concentrations (up to 20% v/v) of glycerol and dimethylsulphoxide stimulate RNA synthesis in vitro, the extent of stimulation being dependent on the nature of the DNA template. This stimulation results mainly from an increase in initiation. The target of these compounds is the DNA template rather than RNA polymerase. Ribosomal RNA synthesis is affected in a temperature‐dependent manner, 20% glycerol lowering the transition temperature between the open and closed forms of the promoter by 4–5°C. Thus in a manner analogous to its effect on gal mRNA synthesis in vitro glycerol partially releases rRNA synthesis from the constraints essential for control.